Glutathione, Glutathione S Transferase Levels and M1/T1 Null Genotype Association with Covid-19 Infection Combined with Diabetes Mellitus Type 2 Patients

Abstract

Background: The companied of covid-19 infection with other disease has been reported to be more influences in infection progressing and development, this regarding the uncontrolling of some cellular processing like oxidative stress mechanisms,

Aim of the study: detection glutathione, glutathione S transferase levels and M1/T1 null genotype association with COVID-19 infection Combined with diabetes mellitus type 2 patients.

Materials and Method: a case control study was implemented to assessment biomarkers using classical lab work procedures and genetic markers using congenital PCR, then association between biomarker and genotyping were implemented.  

Results: The results showed that there were significant differences among study groups in age, duration, FBG, HbA1c and IS, The GSTase level showed a non-significant elevation in DM and covid-19 infected patients, non-significant GSH level was decreased in DM and covid-19 infection groups than control groups, The correlation between GSH and GSTase with study parameters showed significant association in FBG, INS, IN with GSTase, in DM-covid-19 but not in other groups. The results indicate that GSTase affected by DM in covid-19 infection cases but GSH didn’t impact. The correlation between GSH and GSTase level showed a significant positive relation in control and covid-19 groups while weak positive relation in DM2-covid-19 group. The GST genotyping including normal and null genotyping showed a non-significant association of null genotyping for GSTT and GSTM genes with study groups (p= 0.755, 0.1773) respectively, null genotyping of both genes (GSTT+GSTM) showed non-significant association with all other genotyping (p=0.6288) among study groups. The association between GSTT, GSTM genotyping and GSH, GSTase level showed non-significant differences in GSTase and GSH levels among study groups.

Conclusion: the present findings concluded that the DM2 has slightly influence in the covid-19 infection cases regarding GSTase, GSH levels and M1/T1 gene polymorphism.