Catalyse Optimal Analytical Condition Properties in Sub-Cellular Tissues of Freshwater Bivalve, Lamellidens marginalis

Abstract

The Optimal test conditions for catalyze activity were determined from the cytosols of the foot, gills and digestive gland of freshwater mussel. Catalase properties were investigated in L. marginalis due to a lack of quantitative data on optimal analytical conditions of catalyze in subcellular fractions of mollusc tissues. The foot, gill, and digestive gland cytosols of mussels have an optimal temperature 45 °C and pH of 7.5 for catalyse activity. The digestive gland had the highest activation energy (R²=0.3371,53.76 KJ/mol), followed by the gill (R²=0.3889,55.56 KJ/mol) and foot (R²=0.4162,56.58 KJ/mol). Some substrates other than H₂O₂ can inhibit catalyse in the present study. Based on the apparent Km values, in L. marginalis the cytosol of the digestive gland was observed at 7.39 mM H₂O₂, gills at 8.91 mM H₂O₂ and foot at 15.11 mM H₂O₂.