New, Rapid and Sensitive Method for Determination of Vitamins B1, B2 and B9 in Mixture by HPLC Method

Authors

  • Yasmeen Nouh Amrajaa Faculty of Pharmacy, Omar Al –Mukhtar University, Libya
  • Anas S. Mohamied Faculty of Pharmacy, Omar Al –Mukhtar University, Libya
  • Taffaha A. Arhouma Chemistry Department, Faculty of Science, Derna University (Al-Guba), Libya
  • Sajeda S. ElGammei Chemistry Department, Faculty of Science, Derna University, Libya
  • Hamad M. Adress Hasan Chemistry Department, Faculty of Science, Omar Al –Mukhtar University, Libya

Keywords:

B1, B2, B9, HPLC

Abstract

In this study, three water-soluble vitamins (thiamine, riboflavin, and folate) were separated and determined using HPLC. This method provides a fast, accurate, and reliable method for their determination, with recoveries ranging from 91% to 108%. The RSD% values ​​of B1, B2 and B9 solutions were 2.9, 2.3 and 4.2% respectively. The results presented in this study confirm that the proposed approach is not prone to failure. They appear to be accurate, and the method has been successfully applied to the determination of vitamins B1, B2, and B9 in the presence of certain intermediates.

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Published

17-05-2024

Issue

Vol. 19 No. 2 (2024)

Section

Articles

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Copyright (c) 2024 TWIST

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

How to Cite

Amrajaa, Y. N., Mohamied, A. S., Arhouma, T. A., ElGammei, S. S., & Hasan, H. M. A. (2024). New, Rapid and Sensitive Method for Determination of Vitamins B1, B2 and B9 in Mixture by HPLC Method. TWIST, 19(2), 312-318. https://twistjournal.net/twist/article/view/238

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