Area Under Curve and Zero Order UV-Spectrophotometric Methods

Development and Validation for the Simultaneous Estimation ofCaffeine and Metformin in the Formulation

Abstract

Caffeine (CAF) and Metformin (MET), two well-known drugs for CNS stimulation and Antidiabetic activity respectively when taken in combination can create wonders for treatment of fibrosarcoma and type-2 diabetes. We have carried out this research to provide a simple, safe, precise, accurate and inexpensive method for simultaneous estimation of CAF and MET in bulk using Area Under Curve (AUC) and Zero Order UV-Spectroscopic (ZOS) methods. The developed method was also validated as per ICH guidelines. Distilled water is chosen as the solvent for both the methods. For Caffeine, area between 251-289.5 nm was employed to measure the AUC and 273 nm was employed to measure the absorbance. For Metformin, area between 224.5-239.5 nm was employed to measure the AUC and 233 nm was employed to measure the absorbance. In both AUC and ZOS method, CAF and MET showed linearity in the concentration range of 4-20 µg/ml and 0.6-3.0 µg/ml respectively. All the validation parameters for CAF and MET by both the methods exhibited % RSD less than 2% which is well within the acceptance range. Overall, the developed and validated Spectroscopic methods can be effectively exercised for the quality control of CAF and MET in formulation.